Umsebenzi:
Ukukhishwa kwe-nucleic acid noma i-realegent yokuhlanza kuyinto ekhethekile eyenzelwe ukwenza lula ukukhishwa, ukucebisa, nokuhlanzwa kwama-nucleic acid kusuka kumasampula ahlukahlukene okuzalwa. Leli thuluzi elibalulekile lidlala indima ebalulekile e-molefanelar biology kanye nokuxilonga ngokunikeza ama-nucleic acid asezingeni eliphakeme asetshenziselwa izinhlelo ezahlukahlukene zokwehla, kufaka phakathi umtholampilo ekutholweni kwe-vitro.
Izici:
Ukukhishwa kwe-nucleic acid: i-realent yenzelwe ngqo ukukhipha ama-nucleic acid, kufaka phakathi i-DNA ne-RNA, kusuka kumasampula e-biological. Isebenzisa amaphrothokholi alungiselelwe ukuze akhulule kahle ama-nucleic acid ngenkathi kulondolozwa ubuqotho babo.
Ukucebisa nokuhlanzwa: Ngaphezu kokukhishwa, i-reagent ingacebisa futhi ihlanze ama-nucleic acid, isusa ukungcola kanye nokungcola okungaphazamisa ukuhlaziya phansi komfula.
Izinzuzo:
Ukuhlanzeka okuphezulu: I-reagent iqinisekisa ukukhishwa kanye nokuhlanjululwa kwama-nucleic acid ama-nucleic aphezulu, akhululwe ezingogazini angathinta ukunemba kwezicelo eziphansi.
Izithelo ezisezingeni eliphakeme: Izimiso ezenzelwe ukukhishwa kanye nezivumelwano zokuhlanza zihlinzeka ngezithelo ezisezingeni eliphakeme ze-nucleic acid, ukuqinisekisa ukuthi kunezinto ezanele zokuhlaziya nokuhlola okuthe xaxa.
Imiphumela engaguquki: amaphrothokholi ajwayelekile kaReagent kanye nezindlela zokulawula ezisezingeni eliphakeme ziholela ekukhishweni okungaguquki nokuvela kabusha kwe-nucleic acid kanye nemiphumela yokuhlanza.
Ukuguquguquka kwamamodeli acacisiwe: ukutholakala kwamamodeli ahlukile okucaciswa, njenge-16, 32, 48, 64, kanye ne-96, izingxenye / ibhokisi eliguqukayo, kuvumela ukusetshenziswa okuguquguqukayo okusekelwe kwivolumu yamasampula acutshunguliwe.
Ukuhanjiswa komsebenzi okuqondisiwe: I-reagent yenza lula ukukhishwa kwe-nucleic acid kanye nenqubo yokuhlanza, ukonga isikhathi nokwenza ukunciphisa ubunzima bezinqubo zelebhu.
Usekela ukuhlolwa kwemitholampilo: Ama-nucleic acid akhishwe futhi ahlanziwe alungele ukuthola umtholampilo ekutholweni kwe-vitro, enika amandla ukuxilongwa okunembile nokuqapha kwezifo ezahlukahlukene.
Ukuzwela okuthuthukisiwe: Ama-nucleic acid asezingeni eliphakeme atholakala kwi-reaglent athuthukise ukuzwela kwemibono ye-dowstream agameri, aqinisekise ukutholwa okuthembekile kokulandelanisa okuqondiwe.
Ingozi yokungcola encishisiwe
I-ADSHT-PRACH: Ukuthola okuthe xaxa, ama-nucleic acid aphezulu aphezulu ngendlela ehambisanayo kunciphisa isidingo sokukhishwa kabusha kanye nokulondolozwa kwezinsizakusebenza ezibalulekile.
Ukusetshenziswa Okuhlanganisiwe: I-reagent ihlangana ngaphandle komsebenzi ekuhambeni komsebenzi womnyango we-pathology, ukuhlinzeka ngamasampula abalulekile e-nucleic acid ngezicelo ezahlukahlukene zokuxilonga nokucwaninga.
